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Carbonic Anhydrase XII / CA12 Antibody, Mouse MAb

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CA12Antibody Product Information
Immunogen:Recombinant Human Carbonic Anhydrase XII / CA12 protein (Catalog#10617-H08H)
Clone ID:07
Ig Type:Mouse IgG1
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Carbonic Anhydrase XII / CA12 (rh Carbonic Anhydrase XII / CA12; Catalog#10617-H08H; NP_001209.1; Met1-Gln291). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
CA12Antibody Usage Guide
Specificity:Human Carbonic Anhydrase XII / CA12
No cross-reactivity in ELISA with
Mouse CA12
Human CA2
Human CA3
Human CA4
Human CA5A
Human CA5B
Human CA8
Human CA9
Human CA10
Human CA13
Human CA14
Application:ICC/IF, IF, FCM

ICC/IF: 10-25 μg/mL

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Carbonic Anhydrase XII / CA12 Antibody, Mouse MAb, Immunofluorescence
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Confocal immunofluorescence staining of Human CA12 in SKBR3 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-Human CA12 monoclonal antibody (15 µg/ml). Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody and countstained with DAPI (blue). Positive staining was localized to cell membrane.
Carbonic Anhydrase XII Antibody, Mouse MAb, Flow cytometric analysis
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Flow cytometric analysis of Human CA12 expression on MCF-7 cells. Cells were stained with purified anti-Human CA12, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. CA12, also known as Car12 and carbonic anhydrase XII, is a type I  membrane enzyme of an N-terminal extracellular catalytic domain, a membrane-spanning α-helix, and a small intracellular C-terminal domain. It is highly expressed in colon, kidney, prostate, intestine and activated lymphocytes and moderately expressed in pancreas, ovary, and testis. Overexpression of the CA12 is observed in certain human cancers and is used as a tumor marker. rmCA12 corresponds to the extracellular domain and has both carbonic anhydrase activity and esterase activity.

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  • Ivanov, S.V. et al., 1998, Proc. Natl. Acad. Sci. USA 95:12596 - 12601.
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    • Carbonic Anhydrase XII / CA12 Antibody, Mouse MAb, Immunofluorescence
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