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STK4 / MST1 Antibody, Mouse MAb

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Human STK4 Antibody Product Information
Immunogen:Recombinant Human STK4 / MST1 protein (Catalog#11533-H07B)
Clone ID:01
Ig Type:Mouse IgG1
Concentration:
Endotoxin:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human STK4 / MST1 (rh STK4 / MST1; Catalog#11533-H07B; Q13043-1; Glu2-Phe487). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Human STK4 Antibody Usage Guide
Specificity:Human STK4 / MST1
Application:ICC/IF, IF, FCM

FCM: 0.5-2 μg/Test

ICC/IF: 10-25 μg/mL

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Human STK4 Antibody IF Application Image
[Click to enlarge image]
Caption:
Immunofluorescence staining of Human STK4 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human STK4 monoclonal antibody (15 µg/ml) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green). Positive staining was localized to nuclear and cytoplasm.
STK4 / MST1 Antibody, Mouse MAb, Immunofluorescence
Human STK4 Antibody FC Application Image
[Click to enlarge image]
Caption:
Flow cytometric analysis of Human STK4 expression on HeLa cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human STK4, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
STK4 / MST1 Antibody, Mouse MAb
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All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"