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ACOX1 / AOX Antibody, Mouse MAb

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Human ACOX1 Antibody Product Information
Immunogen:Recombinant Human ACOX1 / AOX protein (Catalog#11266-H07B)
Clone ID:09
Ig Type:Mouse IgG1
Concentration:
Endotoxin:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ACOX1 / AOX (rh ACOX1 / AOX; Catalog#11266-H07B; AAH08767.1; Met1-Leu660). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Human ACOX1 Antibody Usage Guide
Specificity:Human ACOX1 / AOX
Application:IHC-P

IHC-P: 20-30 μg/mL

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Human ACOX1 Antibody IHC Application Image
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Caption:
Immunochemical staining of human ACOX1 in human kidney with mouse monoclonal antibody (20 µg/mL, formalin-fixed paraffin embedded sections).
ACOX1 / AOX Antibody, Mouse MAb, Immunochemistry
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Caption:
Immunochemical staining of human ACOX1 in human liver with mouse monoclonal antibody (20 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to hepatocyte.
ACOX1 / AOX Antibody, Mouse MAb, Immunochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of human ACOX1 in human stomach with mouse monoclonal antibody (20 µg/mL, formalin-fixed paraffin embedded sections).
ACOX1 / AOX Antibody, Mouse MAb, Immunochemistry
Other ACOX1 Antibody Products
ACOX1 / AOX Background

Peroxisomal acyl-coenzyme A oxidase 1(ACOX1 or AOX) is the first enzyme of the fatty acid beta-oxidation pathway and belongs to the Acyl-CoA oxidase family. Human liver peroxisomes contain two acyl-CoA oxidases, namely, palmitoyl-CoA oxidase (ACOX1/AOX) and a branched chain acyl-CoA oxidase. The palmitoyl-CoA oxidase (ACOX1/AOX) oxidizes the CoA esters of straight chain fatty acids and prostaglandins and donates electrons directly to molecular oxygen, thereby producing H2O2. Human ACOX1/AOX is a protein of 661-amino acids, including the carboxyl-terminal sequence(Ser-Lys-Leu) known as a minimal peroxisome-targeting signal. Human ACOX1/AOX, the first and rate-limiting enzyme of the peroxisomal β-oxidation pathway, has two isoforms including ACOX1a and ACOX1b, transcribed from a single gene. The human ACOX1b isoform is more effective than the ACOX1a isoform in reversing the Acox1 null phenotype in the mouse partly because of the Substrate utilization differences.

Human ACOX1 / AOX References
  • Vluggens A, et al. (2010) Functional significance of the two ACOX1 isoforms and their crosstalks with PPAR alpha and RXR alpha. Laboratory Investigation. 90: 696-708.
  • Chu R, et al. (1995) Overexpression and characterization of the human peroxisomal acyl-CoA oxidase in insect cells. J Biol Chem. 270 (9): 4908-15.
  • Aoyama T, et al. (1994) Molecular cloning and functional expression of a human peroxisomal acyl-coenzyme A oxidase. Biochem Biophys Res Commun. 198 (3): 1113-8.
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