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ACOX1 / AOX Antibody, Rabbit MAb

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ACOX1Antibody Product Information
Immunogen:Recombinant Human ACOX1 / AOX protein (Catalog#11266-H07B)
Clone ID:003
Ig Type:Rabbit IgG
Concentration:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was obtained from a rabbit immunized with purified, recombinant Human ACOX1 / AOX (rh ACOX1 / AOX; Catalog#11266-H07B; AAH08767.1; Met1-Leu660).
ACOX1Antibody Usage Guide
Specificity:Human ACOX1 / AOX
Application:IHC-P

Immunochemical staining of human ACOX1 in human liver with rabbit monoclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to hepatocyte.

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Images
ACOX1 / AOX Antibody, Rabbit MAb, Immunochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of human ACOX1 in human liver with rabbit monoclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to hepatocyte.
ACOX1 / AOX Antibody, Rabbit MAb, Immunochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of human ACOX1 in human kidney with rabbit monoclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to proximal tubule.
Clusterin / Apolipoprotein J / Apo-J / CLU Antibody, Rabbit MAb, Immunochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of human CLU in human appendix with rabbit monoclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections).
Background

Peroxisomal acyl-coenzyme A oxidase 1(ACOX1 or AOX) is the first enzyme of the fatty acid beta-oxidation pathway and belongs to the Acyl-CoA oxidase family. Human liver peroxisomes contain two acyl-CoA oxidases, namely, palmitoyl-CoA oxidase (ACOX1/AOX) and a branched chain acyl-CoA oxidase. The palmitoyl-CoA oxidase (ACOX1/AOX) oxidizes the CoA esters of straight chain fatty acids and prostaglandins and donates electrons directly to molecular oxygen, thereby producing H2O2. Human ACOX1/AOX is a protein of 661-amino acids, including the carboxyl-terminal sequence(Ser-Lys-Leu) known as a minimal peroxisome-targeting signal. Human ACOX1/AOX, the first and rate-limiting enzyme of the peroxisomal β-oxidation pathway, has two isoforms including ACOX1a and ACOX1b, transcribed from a single gene. The human ACOX1b isoform is more effective than the ACOX1a isoform in reversing the Acox1 null phenotype in the mouse partly because of the Substrate utilization differences.

References
  • Vluggens A, et al. (2010) Functional significance of the two ACOX1 isoforms and their crosstalks with PPAR alpha and RXR alpha. Laboratory Investigation. 90: 696-708.
  • Chu R, et al. (1995) Overexpression and characterization of the human peroxisomal acyl-CoA oxidase in insect cells. J Biol Chem. 270 (9): 4908-15.
  • Aoyama T, et al. (1994) Molecular cloning and functional expression of a human peroxisomal acyl-coenzyme A oxidase. Biochem Biophys Res Commun. 198 (3): 1113-8.
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    Catalog:11266-R003-100
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    • ACOX1 / AOX Antibody, Rabbit MAb, Immunochemistry
    • ACOX1 / AOX Antibody, Rabbit MAb, Immunochemistry
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