|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Recombinant Human Isovaleryl-CoA dehydrogenase / IVD protein (Catalog#14206-H07B)|
|0.2 μm filtered solution in PBS with 5% trehalose|
|This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Isovaleryl-CoA dehydrogenase / IVD (rh Isovaleryl-CoA dehydrogenase / IVD; Catalog#14206-H07B; AAH17202.1; His30-His423). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.|
|Human Isovaleryl-CoA dehydrogenase / IVD|
No cross-reactivity in ELISA with
Insect cell lysate
|WB, ELISA, ICC/IF, IF|
WB: 5-10 μg/mL
ELISA: 0.5-1 μg/mL
This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human Isovaleryl-CoA dehydrogenase / IVD. The detection limit for Human Isovaleryl-CoA dehydrogenase / IVD is approximately 0.078 ng/well.
ICC/IF: 10-25 μg/mL
|This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.|
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Isovaleryl-CoA dehydrogenase, also known as IVD, plays an essential role in processing proteins obtained from the diet. The body breaks down proteins from food into smaller parts called amino acids. Amino acids can be further processed to provide energy for growth and development. Isovaleryl-CoA dehydrogenase helps process a particular amino acid called leucine. Specifically, isovaleryl-CoA dehydrogenase is responsible for the third step in the breakdown of leucine. This step is a chemical reaction that converts a molecule called isovaleryl-CoA to another molecule, 3-methylcrotonyl-CoA. Additional chemical reactions convert 3-methylcrotonyl-CoA into molecules that are used for energy.