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GST Tag Antibody, Mouse MAb

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GSTAntibody Product Information
Immunogen:Recombinant GST protein
Clone ID:6C10G4
Ig Type:Mouse IgG1
Concentration:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Glutathione S-transferase (GST). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
GSTAntibody Usage Guide
Specificity:Recognize GST Tag in fusion proteins.
No cross-reactivity with E.coli cell lysate in ELISA.
Application:WB

WB: 1/1000-1/10000

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Background

Genetic engineers have used glutathione S-transferase to create the GST gene fusion system. This system is used to purify and detect proteins of interest. In a GST gene fusion system, the GST sequence is incorporated into an expression vector alongside the gene sequence encoding the protein of interest. Induction of protein expression from the vector's promoter results in expression of a fusion protein: the protein of interest fused to the GST protein. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. GST is commonly used to create fusion proteins. The tag has the size of 220 amino acids (roughly 26 KDa), which, compared to other tags like the myc- or the FLAG-tag, is quite big. However, many commercially-available sources of GST-tagged plasmids include a thrombin domain for cleavage of the GST tag during protein purification.

References
  • Douglas KT . et al., 1987, Adv Enzymol Relat Areas Mol Biol.?59: 103- 67.?
  • Beckett GJ. et al., 1993, ?Adv Clin Chem. 30: 281-380.
  • Wilce MC. et al., 1994, ?Biochim Biophys Acta. 1205?(1): 1-18.
  • Leaver MJ. et al., 1998, ?Marine Environmental Research. 46?(1-5): 71-4.
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Catalog:11213-MM01-100
List Price: $290.00  (Save $0.00)
Price:$290.00      [How to order]
AvailabilityIn Stock
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  • GST Tag Antibody, Mouse MAb, Western blot
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