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大鼠 TUBB3 基因全长ORF克隆

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Rat TUBB3 cDNA Clone Product Information
NCBI RefSeq:NM_139254.2
RefSeq ORF Size:1353bp
cDNA Description:Full length Clone DNA of Rattus norvegicus tubulin, beta 3 class III.
Gene Synonym:Tubb3
Species:Rat
Vector:pUC19 Vector
Plasmid:cpUC19-ratTubb3
Restriction Site:
Tag Sequence:
Sequence Description:Identical with the Gene Bank Ref. ID sequence except for the point mutations: 654T>C not causing the amino acid variation; 899T>G (M300R) and 974A>G (E325G).
Sequencing primers:M13-47 and RV-M
Promoter:
Application:
Antibiotic in E.coli:Ampicilin
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at room temperature for three months.
Rat TUBB3 Gene Plasmid Map
Rat TUBB3 Gene cDNA Clone (full-length ORF Clone)
pUC19 vector Vector Information:

pUC19 is a small, high-copy number E. coli plasmid cloning vector, of which multiple cloning sites as shown below. The molecule is a small double-stranded circle, 2686 base pairs in length. pUC19 encodes the N-terminal fragment of b-galactosidase (lacZa), which allows for blue/white colony screening (i.e., a-complementation), as well as a pUC origin of replication.

pUC19 vector Usage Suggestion:

The coding sequence can be amplified by PCR with M13-47 and RV-M primers.

Vector Sequence Download
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Catalog: RG80969-U
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  • Rat TUBB3 Gene cDNA Clone (full-length ORF Clone)
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