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pUC19 is a small, high-copy number E. coli plasmid cloning vector, of which multiple cloning sites as shown below. The molecule is a small double-stranded circle, 2686 base pairs in length. pUC19 encodes the N-terminal fragment of b-galactosidase (lacZa), which allows for blue/white colony screening (i.e., a-complementation), as well as a pUC origin of replication.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|Rat CPB2 ORF mammalian expression plasmid, C-GFPSpark tag||RG80947-ACG|
|Rat CPB2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80947-ACR|
|Rat CPB2 ORF mammalian expression plasmid, C-Flag tag||RG80947-CF|
|Rat CPB2 ORF mammalian expression plasmid, C-His tag||RG80947-CH|
|Rat CPB2 ORF mammalian expression plasmid, C-Myc tag||RG80947-CM|
|Rat CPB2 ORF mammalian expression plasmid, C-HA tag||RG80947-CY|
|Rat CPB2 ORF mammalian expression plasmid, N-Flag tag||RG80947-NF|
|Rat CPB2 ORF mammalian expression plasmid, N-His tag||RG80947-NH|
|Rat CPB2 ORF mammalian expression plasmid, N-Myc tag||RG80947-NM|
|Rat CPB2 ORF mammalian expression plasmid, N-HA tag||RG80947-NY|
|Rat CPB2 Gene cDNA clone plasmid||RG80947-U|
|Rat CPB2 natural ORF mammalian expression plasmid||RG80947-UT|
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Carboxypeptidase B2, also known as Carboxypeptidase U, Thrombin-activable fibrinolysis inhibitor, Plasma carboxypeptidase B, CPB2, is a secreted protein which belongs to the peptidase M14 family. Carboxypeptidases are enzymes that hydrolyze C-terminal peptide bonds. The carboxypeptidase family includes metallo-, serine, and cysteine carboxypeptidases. According to their substrate specificity, these enzymes are referred to as carboxypeptidase A (cleaving aliphatic residues) or carboxypeptidase B (cleaving basic amino residues). CPB2 is activated by thrombin and acts on carboxypeptidase B substrates. After thrombin activation, the mature protein downregulates fibrinolysis. CPB2 is synthesized by the liver and circulates in the plasma as a plasminogen-bound zymogen. When it is activated by proteolysis at residue Arg92 by the thrombin / thrombomodulin complex. CPB2 cleaves C-terminal arginine or lysine residues from biologically active peptides such as kinins or anaphylatoxins in the circulation thereby regulating their activities. CPB2 exhibits carboxypeptidase activity and activated CPB2 reduces fibrinolysis by removing the fibrin C-terminal residues that are important for the binding and activation of plasminogen.