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|Recombinant Mouse Alkaline Phosphatase / ALPL protein (Catalog#51134-M08H)|
|0.2 μm filtered solution in PBS|
|Produced in rabbits immunized with purified, recombinant Mouse Alkaline Phosphatase / ALPL (rh Alkaline Phosphatase / ALPL; Catalog#51134-M08H; BAH03518.1; Met1-Ser502). Alkaline Phosphatase / ALPL specific IgG was purified by Mouse Alkaline Phosphatase / ALPL affinity chromatography.|
|Mouse Alkaline Phosphatase / ALPL|
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse Alkaline Phosphatase / ALPL. The detection limit for Mouse Alkaline Phosphatase / ALPL is < 0.039 ng/well.
|This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.|
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Alkaline phosphatase (ALPL) is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. The process of removing the phosphate group is called dephosphorylation. As the name suggests, alkaline phosphatases are most effective in an alkaline environment. It is sometimes used synonymously as basic phosphatase. Alkaline phosphatases (APs) are ubiquitous in many species, from bacteria to human. Four genes encode AP isoenzymes in humans and rodents. Three AP genes are expressed in a tissue-specific manner (i.e., placental, embryonic, and intestinal AP isoenzymes). Expression of the fourth AP gene is nonspecific to a single tissue and is especially abundant in bone, liver, and kidney. This isoenzyme is also called tissue-nonspecific alkaline phosphatase (TNAP). The enzyme tissue non-specific alkaline phosphatase (TNAP) belongs to the ectophosphatase family. TNAP is present in large amounts in bone in which it plays a role in mineralization.