|Datasheet||Specific Preferences||Reviews||Research Topics||Protocols|
|Recombinant Canine PD1 / PDCD1 / CD279 protein (Catalog#70109-D08H)|
|0.2 μm filtered solution in PBS|
|Produced in rabbits immunized with purified, recombinant Canine PD1 / PDCD1 / CD279 (rh PD1 / PDCD1 / CD279; Catalog#70109-D08H; XP_543338.3; Met1-Leu169). PD1 / PDCD1 / CD279 specific IgG was purified by Canine PD1 / PDCD1 / CD279 affinity chromatography.|
|Canine PD1 / PDCD1 / CD279|
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Canine PD1 / PDCD1 / CD279. The detection limit for Canine PD1 / PDCD1 / CD279 is < 0.039 ng/well.
|This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.|
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Programmed cell death 1, also known as PDCD1, is a type I transmembrane glycoprotein, and is an immunoreceptor belonging to the CD28/CTLA-4 family negatively regulates antigen receptor signaling by recruiting protein tyrosine phosphatase, SHP-2 upon interacting with either of two ligands, PD-L1 or PD-L2. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. PD1 has been suggested to be involved in lymphocyte clonal selection and peripheral tolerance, and thus contributes to the prevention of autoimmune diseases. Furthermore, PD1 is shown to be a regulator of virus-specific CD8+ T cell survival in HIV infection. As a cell surface molecule, PDCD1 regulates the adaptive immune response. Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function.