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UBE2W Antibody, Rabbit PAb, Antigen Affinity Purified

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Human UBE2W Antibody Product Information
Immunogen:Recombinant Human UBE2W protein (Catalog#12725-H07E)
Clone ID:
Ig Type:Rabbit IgG
Formulation:0.2 μm filtered solution in PBS
Preparation:Produced in rabbits immunized with purified, recombinant Human UBE2W (rh UBE2W; Catalog#12725-H07E; Q96B02-12; Met1-Cys151). UBE2W specific IgG was purified by Human UBE2W affinity chromatography.
Human UBE2W Antibody Usage Guide
Specificity:Human UBE2W

ELISA: 0.1-0.2 μg/mL

This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human UBE2W. The detection limit for Human UBE2W is < 0.039 ng/well.

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Other UBE2W Antibody Products
UBE2W Background

Ubiquitin-conjugating enzymes, also known as UBE2W, E2 enzymes and more rarely as ubiquitin-carrier enzymes, perform the second step of protein ubiquitination. The modification of protein with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2W is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. It accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. It also catalyzes monoubiquitination and "Lys-11"-linked polyubiquitination. UBE2W is also considered to regulate FANCD2 monoubiquitination. UBE2W exhibits ubiquitin conjugating enzyme activity and catalyzes the monoubiquitination of PHD domain of Fanconi anemia complementation group L (FANCL). Over-expression of UBE2W in cells promotes the monoubiquitination of FANCD2 and down-regulated UBE2W markedly reduces the UV irradiation-induced but not MMC-induced FANCD2 monoubiquitination.

Human UBE2W References
  • Zhang Y, et al. (2008) Generation of mouse UBE2W antibody and analysis of UBE2W expression in mouse tissues. Sheng Wu Gong Cheng Xue Bao. 24(4): 547-52.
  • Scaglione KM, et al. (2011) Ube2w and ataxin-3 coordinately regulate the ubiquitin ligase CHIP. Mol Cell. 43(4): 599-612.
  • Zhang Y, et al. (2011) UBE2W interacts with FANCL and regulates the monoubiquitination of Fanconi anemia protein FANCD2. Mol Cells. 31(2): 113-22.
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