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GFP Antibody, Rabbit PAb, Antigen Affinity Purified

DatasheetSpecific PreferencesReviewsResearch TopicsProtocols
GFPAntibody Product Information
Antigen:Recombinant Aequorea victoria GFP protein (Catalog#13105-S07E)
Clone ID:
Ig Type:Rabbit IgG
Concentration:
Formulation:0.2 μm filtered solution in PBS
Preparation:Produced in rabbits immunized with purified, recombinant Aequorea victoria GFP (rh GFP; Catalog#13105-S07E; AAB65663; Ser2-Lys238). GFP specific IgG was purified by Aequorea victoria GFP affinity chromatography.
GFPAntibody Usage Guide
Specificity:Aequorea victoria GFP
Application:ELISA

ELISA: 0.1-0.2 μg/mL

This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Aequorea victoria GFP. The detection limit for Aequorea victoria GFP is 0.039 ng/well.

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

The green fluorescent protein (GFP) is a protein that exhibit bright green fluorescence when exposed to blue light. The protein is in the shape of a cylinder, comprising 11 strands of beta-sheet with an alpha-helix inside and short helical segments on the ends of the cylinder. Inward-facing sidechains of the barrel induce specific cyclization reactions in the tripeptide Ser65-Tyr66-Gly67 that lead to chromophore formation. Its amazing ability to generate a highly visible, efficiently emitting internal fluorophore is both intrinsically fascinating and tremendously valuable. The green-fluorescent protein (GFP) of the jellyfish Aequorea victoria has always been used as a universal reporter in a broad range of heterologous living cells and organisms. GFP has become well established as a marker of gene expression and protein targeting in intact cells and organisms. 

Reference
  • Chiu WL, et al. (1996) Engineered GFP as a vital reporter in plants. Current biology. 6 (3): 325-30.
  • Pollok BA, et al. (1999) Using GFP in FRET-based applications. Trends in Cell Biology. 9 (2): 57-60.
  • Haseloff J, et al. (1995) GFP in plants . Trends in Genetics. 11 (8): 328-9.
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