|Datasheet||Specific Preferences||Reviews||Research Topics||Protocols|
|Recombinant Aequorea victoria GFP protein (Catalog#13105-S07E)|
|0.2 μm filtered solution in PBS|
|Produced in rabbits immunized with purified, recombinant Aequorea victoria GFP (rh GFP; Catalog#13105-S07E; AAB65663; Ser2-Lys238). GFP specific IgG was purified by Aequorea victoria GFP affinity chromatography.|
|Aequorea victoria GFP|
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Aequorea victoria GFP. The detection limit for Aequorea victoria GFP is 0.039 ng/well.
|This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.|
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
The green fluorescent protein (GFP) is a protein that exhibit bright green fluorescence when exposed to blue light. The protein is in the shape of a cylinder, comprising 11 strands of beta-sheet with an alpha-helix inside and short helical segments on the ends of the cylinder. Inward-facing sidechains of the barrel induce specific cyclization reactions in the tripeptide Ser65-Tyr66-Gly67 that lead to chromophore formation. Its amazing ability to generate a highly visible, efficiently emitting internal fluorophore is both intrinsically fascinating and tremendously valuable. The green-fluorescent protein (GFP) of the jellyfish Aequorea victoria has always been used as a universal reporter in a broad range of heterologous living cells and organisms. GFP has become well established as a marker of gene expression and protein targeting in intact cells and organisms.