|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Mouse CASQ1 ORF mammalian expression plasmid, C-GFPSpark tag||MG53155-ACG|
|Mouse CASQ1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG53155-ACR|
|Mouse CASQ1 ORF mammalian expression plasmid, C-Flag tag||MG53155-CF|
|Mouse CASQ1 ORF mammalian expression plasmid, C-His tag||MG53155-CH|
|Mouse CASQ1 ORF mammalian expression plasmid, C-Myc tag||MG53155-CM|
|Mouse CASQ1 ORF mammalian expression plasmid, C-HA tag||MG53155-CY|
|Mouse CASQ1 Gene cDNA clone plasmid||MG53155-G|
|Mouse CASQ1 ORF mammalian expression plasmid, N-Flag tag||MG53155-NF|
|Mouse CASQ1 ORF mammalian expression plasmid, N-His tag||MG53155-NH|
|Mouse CASQ1 ORF mammalian expression plasmid, N-Myc tag||MG53155-NM|
|Mouse CASQ1 ORF mammalian expression plasmid, N-HA tag||MG53155-NY|
|Mouse CASQ1 natural ORF mammalian expression plasmid||MG53155-UT|
|Learn more about expression Vectors|
Calsequestrin-1 is an isoform of calsequestrin. Calsequestrin is a calcium-binding protein of the sarcoplasmic reticulum. It helps hold calcium in the cisterna of the sarcoplasmic reticulum after a muscle contraction, even though the concentration of calcium in the sarcoplasmic reticulum is much higher than in the cytosol. Two forms of calsequestrin have been identified: Calsequestrin-2 and Calsequestrin-1. Calsequestrin-1 is found in fast skeletal muscle. The release of calsequestrin-bound calcium (through a calcium release channel) triggers muscle contraction. The active protein is not highly structured, more than 50% of it adopting a random coil conformation. When calcium binds there is a structural change whereby the alpha-helical content of the protein increases from 3 to 11%. Both forms of calsequestrin are phosphorylated by casein kinase 2, but the cardiac form is phosphorylated more rapidly and to a higher degree. Calsequestrin-1 is also secreted in the gut where it deprives bacteria of calcium ions.