|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse CASQ1 ORF mammalian expression plasmid, C-GFPSpark tag||MG53155-ACG|
|Mouse CASQ1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG53155-ACR|
|Mouse CASQ1 ORF mammalian expression plasmid, C-Flag tag||MG53155-CF|
|Mouse CASQ1 ORF mammalian expression plasmid, C-His tag||MG53155-CH|
|Mouse CASQ1 ORF mammalian expression plasmid, C-Myc tag||MG53155-CM|
|Mouse CASQ1 ORF mammalian expression plasmid, C-HA tag||MG53155-CY|
|Mouse CASQ1 Gene cDNA clone plasmid||MG53155-G|
|Mouse CASQ1 ORF mammalian expression plasmid, N-Flag tag||MG53155-NF|
|Mouse CASQ1 ORF mammalian expression plasmid, N-His tag||MG53155-NH|
|Mouse CASQ1 ORF mammalian expression plasmid, N-Myc tag||MG53155-NM|
|Mouse CASQ1 ORF mammalian expression plasmid, N-HA tag||MG53155-NY|
|Mouse CASQ1 natural ORF mammalian expression plasmid||MG53155-UT|
|Learn more about expression Vectors|
Calsequestrin-1 is an isoform of calsequestrin. Calsequestrin is a calcium-binding protein of the sarcoplasmic reticulum. It helps hold calcium in the cisterna of the sarcoplasmic reticulum after a muscle contraction, even though the concentration of calcium in the sarcoplasmic reticulum is much higher than in the cytosol. Two forms of calsequestrin have been identified: Calsequestrin-2 and Calsequestrin-1. Calsequestrin-1 is found in fast skeletal muscle. The release of calsequestrin-bound calcium (through a calcium release channel) triggers muscle contraction. The active protein is not highly structured, more than 50% of it adopting a random coil conformation. When calcium binds there is a structural change whereby the alpha-helical content of the protein increases from 3 to 11%. Both forms of calsequestrin are phosphorylated by casein kinase 2, but the cardiac form is phosphorylated more rapidly and to a higher degree. Calsequestrin-1 is also secreted in the gut where it deprives bacteria of calcium ions.