|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse DPEP1 ORF mammalian expression plasmid, C-GFPSpark tag||MG53160-ACG|
|Mouse DPEP1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG53160-ACR|
|Mouse DPEP1 ORF mammalian expression plasmid, C-Flag tag||MG53160-CF|
|Mouse DPEP1 ORF mammalian expression plasmid, C-His tag||MG53160-CH|
|Mouse DPEP1 ORF mammalian expression plasmid, C-Myc tag||MG53160-CM|
|Mouse DPEP1 ORF mammalian expression plasmid, C-HA tag||MG53160-CY|
|Mouse DPEP1 Gene cDNA clone plasmid||MG53160-G|
|Mouse DPEP1 ORF mammalian expression plasmid, N-Flag tag||MG53160-NF|
|Mouse DPEP1 ORF mammalian expression plasmid, N-His tag||MG53160-NH|
|Mouse DPEP1 ORF mammalian expression plasmid, N-Myc tag||MG53160-NM|
|Mouse DPEP1 ORF mammalian expression plasmid, N-HA tag||MG53160-NY|
|Mouse DPEP1 natural ORF mammalian expression plasmid||MG53160-UT|
|Learn more about expression Vectors|
Dehydropeptidase-I, also known as DPEP1, is a kidney membrane enzyme. Its expression in normal colonic mucosa is very low, but it is highly expressed in colorectal adenoma and cancer specimens and is negatively correlated with parameters of pathological aggressiveness and poor prognosis. The overexpression of DPEP1 suppressed tumor cells invasiveness and increased sensitivity to chemotherapeutic agent Gemcitabine. Growth factor EGF treatment decreased DPEP1 expression. Dehydropeptidase-I may be a candidate target in PDAC for designing improved treatments. It uses zinc as a cofactor and acts as a disulfide-linked homodimer.