|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Rhesus TNFSF11 ORF mammalian expression plasmid, C-GFPSpark tag||CG90301-ACG|
|Rhesus TNFSF11 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||CG90301-ACR|
|Rhesus TNFSF11 ORF mammalian expression plasmid, C-Flag tag||CG90301-CF|
|Rhesus TNFSF11 ORF mammalian expression plasmid, C-His tag||CG90301-CH|
|Rhesus TNFSF11 ORF mammalian expression plasmid, C-Myc tag||CG90301-CM|
|Rhesus TNFSF11 ORF mammalian expression plasmid, C-HA tag||CG90301-CY|
|Rhesus TNFSF11 Gene cDNA clone plasmid||CG90301-G|
|Rhesus TNFSF11 ORF mammalian expression plasmid, N-Flag tag||CG90301-NF|
|Rhesus TNFSF11 ORF mammalian expression plasmid, N-His tag||CG90301-NH|
|Rhesus TNFSF11 ORF mammalian expression plasmid, N-Myc tag||CG90301-NM|
|Rhesus TNFSF11 ORF mammalian expression plasmid, N-HA tag||CG90301-NY|
|Rhesus TNFSF11 natural ORF mammalian expression plasmid||CG90301-UT|
|Learn more about expression Vectors|
Tumor necrosis factor ligand superfamily member 11, also known as Receptor activator of nuclear factor kappa-B ligand, Osteoprotegerin ligand, TNFSF11, RANKL, TRANCE, OPGL and CD254, is a single-pass type II membrane protein which belongs to the tumor necrosis factor family. The receptor activator of nuclear factor-kappaB ligand (RANKL), its cognate receptor RANK, and its natural decoy receptor osteoprotegerin have been identified as the final effector molecules of osteoclastic bone resorption. RANK and RANKL are key regulators of bone remodeling and regulate T cell/dendritic cell communications, and lymph node formation. Moreover, RANKL and RANK are expressed in mammary gland epithelial cells and control the development of a lactating mammary gland during pregnancy. Genetically, RANKL and RANK are essential for the development and activation of osteoclasts and bone loss in response to virtually all triggers tested. Inhibition of RANKL function via the natural decoy receptor osteoprotegerin (OPG, TNFRSF11B) prevents bone loss in postmenopausal osteoporosis and cancer metastases. Importantly, RANKL appears to be the pathogenetic principle that causes bone and cartilage destruction in arthritis. RANK-RANKL signaling not only activates a variety of downstream signaling pathways required for osteoclast development, but crosstalk with other signaling pathways also fine-tunes bone homeostasis both in normal physiology and disease. In addition, RANKL and RANK have essential roles in lymph node formation, establishment of the thymic microenvironment, and development of a lactating mammary gland during pregnancy.