|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Rhesus IL12A ORF mammalian expression plasmid, C-GFPSpark tag||CG90290-ACG|
|Rhesus IL12A ORF mammalian expression plasmid, C-OFPSpark / RFP tag||CG90290-ACR|
|Rhesus IL12A ORF mammalian expression plasmid, C-Flag tag||CG90290-CF|
|Rhesus IL12A ORF mammalian expression plasmid, C-His tag||CG90290-CH|
|Rhesus IL12A ORF mammalian expression plasmid, C-Myc tag||CG90290-CM|
|Rhesus IL12A ORF mammalian expression plasmid, C-HA tag||CG90290-CY|
|Rhesus IL12A Gene cDNA clone plasmid||CG90290-G|
|Rhesus IL12A ORF mammalian expression plasmid, N-Flag tag||CG90290-NF|
|Rhesus IL12A ORF mammalian expression plasmid, N-His tag||CG90290-NH|
|Rhesus IL12A ORF mammalian expression plasmid, N-Myc tag||CG90290-NM|
|Rhesus IL12A ORF mammalian expression plasmid, N-HA tag||CG90290-NY|
|Rhesus IL12A natural ORF mammalian expression plasmid||CG90290-UT|
|Learn more about expression Vectors|
Interleukin-12 subunit alpha (IL12A/IL-12p35) is also known as Cytotoxic lymphocyte maturation factor 35 kDa subunit, cytotoxic lymphocyte maturation factor 1, p35, NK cell stimulatory factor chain 1, and interleukin-12 alpha chain. IL12A/IL-12p35 is a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. IL12A/IL-12p35 is required for the T-cell-independent induction of IFN-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. In clinical, IL-12 remains a very promising immunotherapeutic agent because recent cancer vaccination studies in animal models and humans have demonstrated its powerful adjuvant properties. The immune modulating characteristics of IL-12 considered responsible for the adjuvant effects, as well as the results of animal and human cancer vaccination studies with IL-12 applied as an adjuvant. IL12A/IL-12p35 indicates a cytokine which is important in the development of prostate cancer.