|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rhesus PDCD1 ORF mammalian expression plasmid, C-GFPSpark tag||KG90305-ACG|
|Rhesus PDCD1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||KG90305-ACR|
|Rhesus PDCD1 ORF mammalian expression plasmid, C-Flag tag||KG90305-CF|
|Rhesus PDCD1 ORF mammalian expression plasmid, C-His tag||KG90305-CH|
|Rhesus PDCD1 ORF mammalian expression plasmid, C-Myc tag||KG90305-CM|
|Rhesus PDCD1 ORF mammalian expression plasmid, C-HA tag||KG90305-CY|
|Rhesus PDCD1 Gene cDNA clone plasmid||KG90305-G|
|Rhesus PDCD1 ORF mammalian expression plasmid, N-Flag tag||KG90305-NF|
|Rhesus PDCD1 ORF mammalian expression plasmid, N-His tag||KG90305-NH|
|Rhesus PDCD1 ORF mammalian expression plasmid, N-Myc tag||KG90305-NM|
|Rhesus PDCD1 ORF mammalian expression plasmid, N-HA tag||KG90305-NY|
|Rhesus PDCD1 natural ORF mammalian expression plasmid||KG90305-UT|
|Learn more about expression Vectors|
Programmed cell death 1, also known as PDCD1, is a type I transmembrane glycoprotein, and is an immunoreceptor belonging to the CD28/CTLA-4 family negatively regulates antigen receptor signaling by recruiting protein tyrosine phosphatase, SHP-2 upon interacting with either of two ligands, PD-L1 or PD-L2. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. PD1 has been suggested to be involved in lymphocyte clonal selection and peripheral tolerance, and thus contributes to the prevention of autoimmune diseases. Furthermore, PD1 is shown to be a regulator of virus-specific CD8+ T cell survival in HIV infection. As a cell surface molecule, PDCD1 regulates the adaptive immune response. Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function.