|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rhesus IL6R ORF mammalian expression plasmid, C-GFPSpark tag||CG90324-ACG|
|Rhesus IL6R ORF mammalian expression plasmid, C-OFPSpark / RFP tag||CG90324-ACR|
|Rhesus IL6R ORF mammalian expression plasmid, C-Flag tag||CG90324-CF|
|Rhesus IL6R ORF mammalian expression plasmid, C-His tag||CG90324-CH|
|Rhesus IL6R ORF mammalian expression plasmid, C-Myc tag||CG90324-CM|
|Rhesus IL6R ORF mammalian expression plasmid, C-HA tag||CG90324-CY|
|Rhesus IL6R Gene cDNA clone plasmid||CG90324-G|
|Rhesus IL6R ORF mammalian expression plasmid, N-Flag tag||CG90324-NF|
|Rhesus IL6R ORF mammalian expression plasmid, N-His tag||CG90324-NH|
|Rhesus IL6R ORF mammalian expression plasmid, N-Myc tag||CG90324-NM|
|Rhesus IL6R ORF mammalian expression plasmid, N-HA tag||CG90324-NY|
|Rhesus IL6R natural ORF mammalian expression plasmid||CG90324-UT|
|Learn more about expression Vectors|
Interleukin 6 receptor (IL-6R) also known as CD126 (Cluster of Differentiation 126) is a type I cytokine receptor. The low concentration of a soluble form of IL-6 receptor (sIL-6R) acts as an agonist of IL-6 activity. In the IL-6R/CD126/IL6R system, both a membrane-bound IL-6R and a sIL-6R protein are able to mediate IL-6 signals into the cells through the interaction of gp130. The resulting IL-6/sIL-6R protein complex is also capable of binding to gp130 and inducing intracellular signalling. Through this so-called 'trans-signalling' mechanism, IL-6 is able to stimulate cells that lack an endogenous mIL-6R. High levels of IL-6 and sIL-6R have been reported in several chronic inflammatory and autoimmune diseases as well as in cancer.