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Human E-Cadherin / CDH1 / E-cad / CD324 ELISA Kit

DatasheetComponentsSpecific ReferencesReviewsRelated Products
Principle Of The Test
Solid Phase Sandwich ELISA
Intended Use
For the quantitative determination of Human E-Cadherin / CDH1 / E-cad / CD324 concentration in serum.
The use of this kit for other sample types need be validated by the end user due to the complexity of natural targets and unpredictable interference.
Assay Range
46.88-3000 pg/mL
The minimum detectable dose of Human E-Cadherin / CDH1 / E-cad / CD324 is typically less than 7.95 pg/mL. The MDD was determined by adding three standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.
Materials Provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
Human CDH1 ELISA Kit Standard Curve
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This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
Human E-Cadherin / CDH1 / E-cad / CD324 ELISA Kit, TYPICAL DATA
Human CDH1 ELISA Kit Specificity
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This assay recognizes both recombinant and natural Human E-cad. The factors listed above were prepared at 2000 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

Human E-Cadherin / CDH1 / E-cad / CD324 ELISA Kit, SPECIFICITY
E-Cadherin/CDH1/E-cad/CD324 Background

Cadherins are calcium-dependent cell adhesion proteins which preferentially interact with themselves in a homophilic manner in connecting cells, and thus may contribute to the sorting of heterogeneous cell type. E-cadherin (E-Cad), also known as CDH1 and CD324, is a calcium-dependent cell adhesion molecule the intact function of which is crucial for the establishment and maintenance of epithelial tissue polarity and structural integrity. Mutations in CDH1 occur in diffuse type gastric cancer, lobular breast cancer, and endometrial cancer. In human cancers, partial or complete loss of E-cadherin expression correlates with malignancy. During apoptosis or with calcium influx, E-Cad is cleaved by the metalloproteinase to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. E-Cad has been identified as a potent invasive suppressor, as downregulation of E-cadherin expression is involved in dysfunction of the cell-cell adhesion system, and often correlates with strong invasive potential and poor prognosis of human carcinomas.

Human E-Cadherin/CDH1/E-cad/CD324 References
  • Wang HD, et al. (2004) CDH1 germline mutation in hereditary gastric carcinoma. World J Gastroenterol. 10(21): 3088-93.
  • Masterson J, et al. (2007) Posttranslational truncation of E-cadherin and significance for tumour progression. Cells Tissues Organs. 185(1-3): 175-9.
  • Mrgineanu E, et al. (2008) Correlation between E-cadherin abnormal expressions in different types of cancer and the process of metastasis. Rev Med Chir Soc Med Nat Iasi. 112(2): 432-6.
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    Catalog: KIT10204-1
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