Quick Order

Text Size:AAA

Human HER2 / ErbB2 ELISA Kit

DatasheetSpecific PreferencesReviewsResearch TopicsProtocols
PRINCIPLE OF THE TEST
Solid Phase Sandwich ELISA
INTENDED USE
For the quantitative determination of Human HER2 / ErbB2 concentration in serum.
The use of this kit for other sample types need be validated by the end user due to the complexity of natural targets and unpredictable interference.
ASSAY RANGE
62.5-4000 pg/mL
Sensitivity
The minimum detectable dose of Human HER2 / ErbB2 is typically less than 7.95 pg/mL. The MDD was determined by adding three standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.
MATERIALS PROVIDED
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
Images
Human HER2 / ErbB2 ELISA Kit, TYPICAL DATA
View larger image
Caption:
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
Human HER2 / ErbB2 ELISA Kit, SPECIFICITY
View larger image
Caption:
This assay recognizes both recombinant and natural Human ErbB2. The factors listed above were prepared at 1250 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

Human epidermal growth factor receptor 2 (HER2), also known as ErbB2, NEU, and CD340, is a type I membrane glycoprotein, and belongs to the epidermal growth factor (EGF) receptor family. HER2 protein cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. However, HER2 forms a heterodimer with other ligand-bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase-mediated activation of downstream molecules. HER2 plays a key role in development, cell proliferation and differentiation. HER2 gene has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on.

Reference
  • Krawczyk N, et al. (2009) HER2 status on persistent disseminated tumor cells after adjuvant therapy may differ from initial HER2 status on primary tumor. Anticancer Res. 29(10): 4019-24.
  • Size / Price
    Catalog:KIT10004-1
    List Price: $320.00  (Save $0.00)
    Price:$320.00      [How to order]
    Availsability:on Sale
    Images
    • Human HER2 / ErbB2 ELISA Kit, TYPICAL DATA
    • Human HER2 / ErbB2 ELISA Kit, SPECIFICITY
    Recently Viewed Items
    Please note:All products are "FOR RESEARCH UES ONLY AND ARE NOT INTENDED FOR DIAGNOSTICE OFR THERAPEUTIC USE"