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Human C1 inhibitor / SerpinG1 ELISA Kit

DatasheetComponentsSpecific ReferencesReviewsRelated Products
Principle Of The Test
Solid Phase Sandwich ELISA
Intended Use
For the quantitative determination of Human C1 inhibitor / SerpinG1 concentration in serum.
The use of this kit for other sample types need be validated by the end user due to the complexity of natural targets and unpredictable interference.
Assay Range
156.25-10000 pg/mL
The minimum detectable dose of Human C1 inhibitor / SerpinG1 is typically less than 7.95 pg/mL. The MDD was determined by adding three standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.
Materials Provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
Human SERPING1 ELISA Kit Standard Curve
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This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
Human C1 inhibitor / SerpinG1 ELISA Kit, TYPICAL DATA
Human SERPING1 ELISA Kit Specificity
[Click to enlarge image]
This assay recognizes both recombinant and natural Human SerpinG1. The factors listed above were prepared at 60 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

Human C1 inhibitor / SerpinG1 ELISA Kit, SPECIFICITY
C1 inhibitor/SerpinG1 Background

Plasma protease C1 inhibitor, also known as C1-inhibiting factor, C1-INH, C1 esterase inhibitor, SERPING1 and C1IN, is a serine proteinase inhibitor (serpin) that regulates activation of both the complement and contact systems. By its C-terminal part (serpin domain), characterized by three beta-sheets and an exposed mobile reactive loop, C1-INH binds, and blocks the activity of its target proteases. The N-terminal end (nonserpin domain) confers to C1-INH the capacity to bind lipopolysaccharides and E-selectin. Owing to this moiety, C1-INH intervenes in regulation of the inflammatory reaction. The heterozygous deficiency of C1-INH results in hereditary angioedema (HAE). Owing to its ability to modulate the contact and complement systems and the convincing safety profile, plasma-derived C1 inhibitor is an attractive therapeutic protein to treat inflammatory diseases other than HAE. Deficiency of C1 inhibitor results in hereditary angioedema, which is characterized by recurrent episodes of localized angioedema of the skin, gastrointestinal mucosa or upper respiratory mucosa. C1 inhibitor may prove useful in a variety of other diseases including septic shock, reperfusion injury, hyperacute transplant rejection, traumatic and hemorrhagic shock, and the increased vascular permeability associated with thermal injury, interleukin-2 therapy and cardiopulmonary bypass.

Human C1 inhibitor/SerpinG1 References
  • Davis AE 3rd. et al. (2004) Biological effects of C1 inhibitor. Drug News Perspect. 17(7): 439-46.
  • Cicardi M, et al. (2005) C1 inhibitor: molecular and clinical aspects. Springer Semin Immunopathol. 27(3): 286-98.
  • Wouters D, et al. (2008) C1 inhibitor: just a serine protease inhibitor? New and old considerations on therapeutic applications of C1 inhibitor. Expert Opin Biol Ther. 8(8): 1225-40.
  • Cugno M, et al. (2009) C1-inhibitor deficiency and angioedema: molecular mechanisms and clinical progress. Trends Mol Med. 15(2): 69-78.
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    Catalog: KIT10995-1
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    • Human C1 inhibitor / SerpinG1 ELISA Kit, TYPICAL DATA
    • Human C1 inhibitor / SerpinG1 ELISA Kit, SPECIFICITY