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Human SPHK1 / Sphingosine Kinase 1 Protein

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Human SPHK1 Protein Product Information
Synonym:SPHK1, SPHK, SPK
Protein Construction:A DNA sequence encoding the human SPHK1 (Q9NYA1-1)(Met1-Leu384) was fused with two additional amino acids (Gly & Pro) at the N-terminus.
Expressed Host:Baculovirus-Insect Cells
Shipping:Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.
Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Human SPHK1 Protein QC Testing
Purity:> 85 % as determined by SDS-PAGE
Bio-Activity:The specific activity was determined to be 1028 nmol/min/mg using Sphingosine Kinase Substrate as substrate.
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Gly
Molecule Mass:The recombinant human SPHK1 consists of 386 amino acids and has a calculated molecular mass of 42.7 kDa. The recombinant protein migrates as an approximately 46 kDa band in SDS-PAGE under reducing conditions.
Formulation:Supplied as sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.0.
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Human SPHK1 Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Other SPHK1 Recombinant Protein Products
SPHK1 / Sphingosine Kinase 1 Background

SPHK1, also known as sphingosine Kinase 1, catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P). S1P exhibits a broad spectrum of biological activities including cell proliferation, survival, migration, cytoskeletal organization, and morphogenesis. It is a ligand for cell surface G protein-coupled receptors. SPHK 1 is a potential therapeutic target for the control of cancer and inflammation. SPHK1 plays a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes.

Human SPHK1 / Sphingosine Kinase 1 References
  • Kohama T. et al., 1998, J Biol Chem. 273 (37): 23722-8.
  • Xia P. et al., 2002, J Biol Chem. 277 (10): 7996-8003.
  • Tsukahara T. et al., 2002, Tanpakushitsu Kakusan Koso. 47 (4): 509-13.
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    Catalog: 15679-HNCB-50
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