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Dromedary camel coronavirus (DcCoV) (strain HKU23-368F) Spike protein ORF mammalian expression plasmid (Codon Optimized)

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DcCoV DcCoV-Spike cDNA Clone Product Information
Gene_bank_ref_id:
RefSeq ORF Size:4101bp
cDNA Description:Full length Clone DNA of Dromedary camel coronavirus (DcCoV) (strain HKU23-368F) Spike protein (S).
Gene Synonym:DcCoV-Spike
Species:DcCoV
Vector:pGEM-T Vector
Plasmid:pGEM-DcCoV/HKU23-AHN64792-S
Restriction Site:
Tag Sequence:
Sequence Description:A number of silent mutations were introduced into the DNA sequence in order to increase its protein expression level in mammalian cell system. The translated amino acid sequence is identical with KF906251.1.
Sequencing primers:SP6 and T7 or M13-47 and RV-M
Promoter:
Application:
Antibiotic in E.coli:
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at ambient temperature for three months.
pGEM-T Vector Information

The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

pGEM-T Simple Usage Suggestion:

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

Vector Sequence Download
Dromedary camel coronavirus (DcCoV) (strain HKU23-368F) Spike protein ORF mammalian expression plasmid (Codon Optimized) on other vectors
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Catalog: VG40457-G
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