|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse CANX ORF mammalian expression plasmid, C-GFPSpark tag||MG53126-ACG|
|Mouse CANX ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG53126-ACR|
|Mouse CANX ORF mammalian expression plasmid, C-Flag tag||MG53126-CF|
|Mouse CANX ORF mammalian expression plasmid, C-His tag||MG53126-CH|
|Mouse CANX ORF mammalian expression plasmid, C-Myc tag||MG53126-CM|
|Mouse CANX ORF mammalian expression plasmid, C-HA tag||MG53126-CY|
|Mouse CANX Gene cDNA clone plasmid||MG53126-G|
|Mouse CANX ORF mammalian expression plasmid, N-Flag tag||MG53126-NF|
|Mouse CANX ORF mammalian expression plasmid, N-His tag||MG53126-NH|
|Mouse CANX ORF mammalian expression plasmid, N-Myc tag||MG53126-NM|
|Mouse CANX ORF mammalian expression plasmid, N-HA tag||MG53126-NY|
|Mouse CANX natural ORF mammalian expression plasmid||MG53126-UT|
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Calnexin is a calcium-binding protein that belongs to the calreticulin family. It interacts with newly synthesized glycoproteins in the endoplasmic reticulum. Calnexin seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse.