|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat F9 ORF mammalian expression plasmid, C-GFPSpark tag||RG81664-ACG|
|Rat F9 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG81664-ACR|
|Rat F9 ORF mammalian expression plasmid, C-Flag tag||RG81664-CF|
|Rat F9 ORF mammalian expression plasmid, C-His tag||RG81664-CH|
|Rat F9 ORF mammalian expression plasmid, C-Myc tag||RG81664-CM|
|Rat F9 ORF mammalian expression plasmid, C-HA tag||RG81664-CY|
|Rat F9 Gene cDNA clone plasmid||RG81664-G|
|Rat F9 ORF mammalian expression plasmid, N-Flag tag||RG81664-NF|
|Rat F9 ORF mammalian expression plasmid, N-His tag||RG81664-NH|
|Rat F9 ORF mammalian expression plasmid, N-Myc tag||RG81664-NM|
|Rat F9 ORF mammalian expression plasmid, N-HA tag||RG81664-NY|
|Rat F9 natural ORF mammalian expression plasmid||RG81664-UT|
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Coagulation factor IX, also known as Christmas factor, Plasma thromboplastin component and PTC, is a secreted protein which belongs to the peptidase S1 family. Coagulation factor IX / F9 contains two EGF-like domains, one Gla (gamma-carboxy-glutamate) domain and one?peptidase S1 domain. Coagulation factor IX / F9 is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca2+ons, phospholipids, and factor VIIIa. Defects in Coagulation factor IX / F9 are the cause of thrombophilia due to factor IX defect which is a hemostatic disorder characterized by a tendency to thrombosis. Defects in Coagulation factor IX / F9 are also the cause of recessive X-linked hemophilia B ( HEMB ) which also known as Christmas disease.