|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat MBL2 ORF mammalian expression plasmid, C-GFPSpark tag||RG81661-ACG|
|Rat MBL2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG81661-ACR|
|Rat MBL2 ORF mammalian expression plasmid, C-Flag tag||RG81661-CF|
|Rat MBL2 ORF mammalian expression plasmid, C-His tag||RG81661-CH|
|Rat MBL2 ORF mammalian expression plasmid, C-Myc tag||RG81661-CM|
|Rat MBL2 ORF mammalian expression plasmid, C-HA tag||RG81661-CY|
|Rat MBL2 Gene cDNA clone plasmid||RG81661-G|
|Rat MBL2 ORF mammalian expression plasmid, N-Flag tag||RG81661-NF|
|Rat MBL2 ORF mammalian expression plasmid, N-His tag||RG81661-NH|
|Rat MBL2 ORF mammalian expression plasmid, N-Myc tag||RG81661-NM|
|Rat MBL2 ORF mammalian expression plasmid, N-HA tag||RG81661-NY|
|Rat MBL2 natural ORF mammalian expression plasmid||RG81661-UT|
|Learn more about expression Vectors|
MBL (mannose-binding lectin) is primarily a liver-derived collagen-like serum protein, which binds sugar structures on micro-organisms and on dying host cells and is one of the four known mediators that initiate activation of the complement system via the lectin pathway. MBL and the ficolins (Ficolin-1, Ficolin-2 and Ficolin-3) are soluble collagen-like proteins that are involved in innate immune defence. They bind sugar structures or acetylated compounds present on microorganisms and on dying host cells and they initiate activation of the lectin complement pathway in varying degrees. MBL2 encodes the mannose-binding lectin, which is a key player in the innate immune system and has recently been found to play a role in development of type 1 diabetes and gestational diabetes mellitus. Common variant alleles situated both in promoter and structural regions of the MBL2 gene influence the stability and the serum concentration of the protein. Several polymorphisms in the promoter and structural regions of MBL2 adversely affect the plasma concentration and oligomeric state of MBL. The possession of mutant alleles has been linked to disease outcome for a variety of bacterial and viral infections. Mutant MBL2 haplotypes have been linked to disease progression and response to therapy in HCV infection.