|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse CANX ORF mammalian expression plasmid, C-GFPSpark tag||MG53126-ACG|
|Mouse CANX ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG53126-ACR|
|Mouse CANX ORF mammalian expression plasmid, C-Flag tag||MG53126-CF|
|Mouse CANX ORF mammalian expression plasmid, C-His tag||MG53126-CH|
|Mouse CANX ORF mammalian expression plasmid, C-Myc tag||MG53126-CM|
|Mouse CANX ORF mammalian expression plasmid, C-HA tag||MG53126-CY|
|Mouse CANX Gene cDNA clone plasmid||MG53126-G|
|Mouse CANX ORF mammalian expression plasmid, N-Flag tag||MG53126-NF|
|Mouse CANX ORF mammalian expression plasmid, N-His tag||MG53126-NH|
|Mouse CANX ORF mammalian expression plasmid, N-Myc tag||MG53126-NM|
|Mouse CANX ORF mammalian expression plasmid, N-HA tag||MG53126-NY|
|Mouse CANX natural ORF mammalian expression plasmid||MG53126-UT|
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Calnexin is a calcium-binding protein that belongs to the calreticulin family. It interacts with newly synthesized glycoproteins in the endoplasmic reticulum. Calnexin seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse.