|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse LCK ORF mammalian expression plasmid, C-GFPSpark tag||MG53117-ACG|
|Mouse LCK ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG53117-ACR|
|Mouse LCK ORF mammalian expression plasmid, N-GFPSpark tag||MG53117-ANG|
|Mouse LCK ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG53117-ANR|
|Mouse LCK ORF mammalian expression plasmid, C-Flag tag||MG53117-CF|
|Mouse LCK ORF mammalian expression plasmid, C-His tag||MG53117-CH|
|Mouse LCK ORF mammalian expression plasmid, C-Myc tag||MG53117-CM|
|Mouse LCK ORF mammalian expression plasmid, C-HA tag||MG53117-CY|
|Mouse LCK Gene cDNA clone plasmid||MG53117-G|
|Mouse LCK ORF mammalian expression plasmid, N-Flag tag||MG53117-NF|
|Mouse LCK ORF mammalian expression plasmid, N-His tag||MG53117-NH|
|Mouse LCK ORF mammalian expression plasmid, N-Myc tag||MG53117-NM|
|Mouse LCK ORF mammalian expression plasmid, N-HA tag||MG53117-NY|
|Mouse LCK natural ORF mammalian expression plasmid||MG53117-UT|
|Learn more about expression Vectors|
Protein kinases are critically involved in signaling pathways that regulate cell growth, differentiation, activation, and survival. Initially identified as a T-cell specific member of the Src family of protein tyrosine kinases, Lck has become the object of intensive investigations which have revealed a key role for this kinase in the central processes controlling T-cell development, activation, proliferation and survival. Lck is expressed specifically in lymphoid cells. It contains one protein kinase domain, one SH2 domain, and one SH3 domain. It is associated with a variety of cell surface receptors and is critical for signal transduction from the T-cell antigen receptor (TCR). Consequently, Lck is targeted by regulatory proteins of T-lymphotropic viruses, especially by the Herpesvirus saimiri (HVS) tyrosine kinase interacting protein (Tip). This oncoprotein physically interacts with Lck in HVS transformed T cells and has an impact on its catalytic activity. Together with the identification of defects in the regulation of Lck expression or activity in T-cell leukemias, suggests that dysregulation of Lck might play a role in neoplastic transformation. However, under certain conditions Lck is also involved in the induction of apoptosis. This chemosensitizing effect of Lck is independent of T-cell receptor signaling and does not require the kinase activity of Lck. The findings demonstrate that Lck might be part of two independent signaling pathways leading to either cell proliferation or apoptosis.