|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse CNPY4 ORF mammalian expression plasmid, C-GFPSpark tag||MG53099-ACG|
|Mouse CNPY4 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG53099-ACR|
|Mouse CNPY4 ORF mammalian expression plasmid, C-Flag tag||MG53099-CF|
|Mouse CNPY4 ORF mammalian expression plasmid, C-His tag||MG53099-CH|
|Mouse CNPY4 ORF mammalian expression plasmid, C-Myc tag||MG53099-CM|
|Mouse CNPY4 ORF mammalian expression plasmid, C-HA tag||MG53099-CY|
|Mouse CNPY4 Gene cDNA clone plasmid||MG53099-G|
|Mouse CNPY4 ORF mammalian expression plasmid, N-Flag tag||MG53099-NF|
|Mouse CNPY4 ORF mammalian expression plasmid, N-His tag||MG53099-NH|
|Mouse CNPY4 ORF mammalian expression plasmid, N-Myc tag||MG53099-NM|
|Mouse CNPY4 ORF mammalian expression plasmid, N-HA tag||MG53099-NY|
|Mouse CNPY4 natural ORF mammalian expression plasmid||MG53099-UT|
|Learn more about expression Vectors|
CNPY4 belongs to the canopy family. CNPY4 interacts with toll-like receptor 4 (TLR4) and plays a role in the regulation of the cell surface expression of TLR4. Toll-like receptors (TLRs) recognize microbial products and induce immune responses. Lipopolysaccharide is recognized by the receptor complex consisting of TLR4 and MD-2. As CNPY4, PRAT4B also regulates cell surface expression of TLR4. PRAT4B has a signal peptide followed by a mature peptide. It is associated with the hypoglycosylated, immature form of TLR4 but not with MD-2 or TLR2.