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MERS-CoV (HCoV-EMC/2012) Spike Protein Antibody, Rabbit MAb

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SpikeAntibody Product Information
Immunogen:Recombinant Novel coronavirus (HCoV-EMC/2012) Spike Protein (ECD, aa 1-1297) (Catalog#40069-V08B)
Ig Type:Rabbit IgG
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was obtained from a rabbit immunized with purified, recombinant recombinant novel coronavirus (HCoV-EMC/2012) Spike Protein (Catalog#40069-V08B; AFS88936.1; Met1-Trp1297).
SpikeAntibody Usage Guide
Specificity:The antibody reacts with Novel coronavirus (HCoV-EMC/2012) full-length Spike protein (S protein).
Has cross-reactivity in ELISA with
MERS-CoV Spike Protein (aa 1-1297, 40069-V08B)
MERS-CoV Spike Protein S1 (aa 1-725, 40069-V08B1)
MERS-CoV Spike Protein RBD (aa 367-606, 40071-V31B1)
No cross-reactivity in ELISA with
MERS-CoV Spike Protein S2 (aa 726-1296, 40070-V08B)
Application:Microneutralizaiton (MN)
Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles
MERS-CoV (HCoV-EMC/2012) Spike Protein Antibody, Rabbit Mab, Neutralization
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Neutralization of MERS-CoV pseudovirus by R723.
Positive Control:293T/DPP4 cells were infected with MERS-CoV pseudovirus;
Negative Control:293T/DPP4 cells without MERS-CoV pseudovirus;
R723: MERS-CoV pseudovirus infection in 293T/DPP4 cells was inhibited by R723.


The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell: they are essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. Most notable is severe acute respiratory syndrome (SARS). The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein alone can mediate the membrane fusion required for virus entry and cell fusion. It is also a major immunogen and a target for entry inhibitors. The SARS-CoV spike (S) protein is composed of two subunits; the S1 subunit contains a receptor-binding domain that engages with the host cell receptor angiotensin-converting enzyme 2 and the S2 subunit mediates fusion between the viral and host cell membranes. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity, during infection with SARS-CoV. 

  • Shen S, et al. (2007) Expression, glycosylation, and modification of the spike (S) glycoprotein of SARS CoV. Methods Mol Biol. 379: 127-35.
  • Du L, et al. (2009) The spike protein of SARS-CoV--a target for vaccine and therapeutic development. Nat Rev Microbiol. 7 (3): 226-36.
  • Xiao X, et al. (2004) The SARS-CoV S glycoprotein. Cell Mol Life Sci. 61 (19-20): 2428-30.
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