|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Recombinant Human Acetoacetyl-CoA Synthetase protein (Catalog#11117-H07B)|
|0.2 μm filtered solution in PBS|
|Produced in rabbits immunized with purified, recombinant Human Acetoacetyl-CoA Synthetase (rh Acetoacetyl-CoA Synthetase; Catalog#11117-H07B; NP_076417.2; Met1-Phe672). Acetoacetyl-CoA Synthetase specific IgG was purified by Human Acetoacetyl-CoA Synthetase affinity chromatography.|
|Human Acetoacetyl-CoA Synthetase|
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human Acetoacetyl-CoA Synthetase. The detection limit for Human Acetoacetyl-CoA Synthetase is < 0.039 ng/well.
|This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.|
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Acetoacetyl-CoA Synthetase (AACS) is a novel cytosolic ketone body (acetoacetate)-specific ligase. The AACS in adipose tissue plays an important role in utilizing ketone body for the fatty acid-synthesis during adipose tissue development. It had been improved that Acetoacetyl-CoA Synthetase is an essential enzyme for the synthesis of fatty acid and cholesterol from ketone bodies, was found to be highly expressed in mouse adipose tissue, and GC box and C/EBPs motif were crucial for AACS promoter activity in 3T3-L1 adipocytes. Moreover, AACS promoter activity was controlled mainly by C/EBPalpha during adipogenesis. AACS gene expression is particularly abundant in white adipose tissue, as it is induced during adipocyte differentiation. The human AACS promoter is a PPARgamma target gene and that this nuclear receptor is recruited to the AACS promoter by direct interaction with Sp1 (stimulating protein-1). The Acetoacetyl-CoA Synthetase has important roles in the regulation of ketone body utilization in rat liver and that these hypocholesterolemic agents have the ability to remedy the impaired utilization of ketone bodies under the diabetic condition.