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Influenza A H3N2 (A/Indiana/07/2012) Hemagglutinin / HA Gene cDNA Clone (Codon Optimized, full-length ORF Clone)

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HAcDNA Clone Product Information
Gene_bank_ref_id:
cDNA Size:1701
cDNA Description:ORF Clone of Influenza A H3N2 (A/Indiana/07/2012) Hemagglutinin DNA.
Gene Synonym:Hemagglutinin, HA
Species:H3N2
Vector:pGEM-T Vector
Restriction Site:
Tag Sequence:
Sequence Description:A number of silent mutations were introduced into the DNA sequence in order to increase its protein expression level in mammalian cell system. The translated amino acid sequence is identical with AFR61003.1.
Shipping_carrier:Each tube contains approximately 10 μg of lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at ambient temperature for three months.
pGEM-T Vector Information

The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

pGEM-T Simple Usage Suggestion:

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

Vector Sequence Download
Influenza A H3N2 (A/Indiana/07/2012) Hemagglutinin / HA Gene cDNA Clone (Codon Optimized, full-length ORF Clone) on other vectors
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Influenza A H3N2 (A/Indiana/07/2012) Hemagglutinin / HA Gene cDNA Clone (Codon Optimized, full-length ORF Clone), expression ready, N-His-taggedVG40395-NH$315
Influenza A H3N2 (A/Indiana/07/2012) Hemagglutinin / HA Gene cDNA Clone (Codon Optimized, full-length ORF Clone), expression ready, N-Myc-taggedVG40395-NM$315
Influenza A H3N2 (A/Indiana/07/2012) Hemagglutinin / HA Gene cDNA Clone (Codon Optimized, full-length ORF Clone), expression ready, N-HA-taggedVG40395-NY$315
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