|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Recombinant Mouse PSGL-1 / CD162 protein (Catalog#50770-MCCH)|
|0.2 μm filtered solution in PBS|
|Produced in rabbits immunized with purified, recombinant Mouse PSGL-1 / CD162 (rh SELPLG; Catalog#50770-MCCH; Q62170; Met1-Cys307). Total IgG was purified by Protein A affinity chromatography.|
|Mouse PSGL-1 / CD162|
ELISA: 0.5-1.0 μg/mL
This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect Mouse PSGL-1 / CD162. The detection limit for Mouse PSGL-1 / CD162 is 0.00245 ng/well.
|This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.|
P-selectin glycoprotein ligand-1 (PSGL-1), also known as SELPLG or CD162, is the high affinitycounter-receptor for P-selectin on expressed on activated endothelial cells and platelets. PSGL-1 is a mucin-type glycoprotein, expressed on leukocytes and platelets as a homodimer of two disulfide-linked subunits of ~120 kD. As cell adhesion molecules, multiple studies have shown that PSGL-1/ P-selectin interaction is required for the normal recruitment of leukocytes during inflammatory reactions, and also participates in hemostatic responses. PSGL-1 protein requires two distinct posttranslational modifications for the Ca2+-dependent recognition by the lectin domain of P-selectin, that is tyrosine sulfation and specific O-linked glycosylation (sialic acid and fucose). PSGL-1 can also bind to other two members of the selectin family, E-selectin (endothelial) and L-selectin (leukocyte), but binds best to P-selectin.
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