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ITGA5 & ITGB1 Antibody, Mouse MAb

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Human ITGA5 & ITGB1 Antibody Product Information
Immunogen:Recombinant Human ITGA5 & ITGB1 protein (Catalog#CT014-H2508H)
Clone ID:358
Ig Type:Mouse IgG1
Concentration:
Endotoxin:
Formulation:0.2 μm filtered solution in PBS
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ITGA5 & ITGB1 (rh ITGA5 & ITGB1; Catalog#CT014-H2508H; P08648 & P05556-1; Met1-Tyr995 & Met1-Asp728). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Human ITGA5 & ITGB1 Antibody Usage Guide
Specificity:Human ITGA5 & ITGB1
Application:ICC/IF, IF

ICC/IF: 10-25 μg/mL

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Human ITGA5 & ITGB1 Antibody IF Application Image
[Click to enlarge image]
Caption:
Immunofluorescence staining of Human ITGA5&ITGB1 in MSC cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human ITGA5&ITGB1 monoclonal antibody (15 µg/ml) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cell membrane.
ITGA5 & ITGB1 Antibody, Mouse MAb, Immunofluorescence
Other ITGA5 & ITGB1 Antibody Products
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"