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CD208 / DC-LAMP / LAMP3 Antibody, Rabbit MAb

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Human LAMP3 Antibody Product Information
Immunogen:Recombinant Human CD208 / DC-LAMP / LAMP3 protein (Catalog#10527-H08H)
Clone ID:007
Ig Type:Rabbit IgG
Formulation:0.2 μm filtered solution in PBS
Preparation:This antibody was obtained from a rabbit immunized with purified, recombinant Human CD208 / DC-LAMP / LAMP3 (rh CD208 / DC-LAMP / LAMP3; Catalog#10527-H08H; NP_055213.2; Lys28-Thr381).
Human LAMP3 Antibody Usage Guide
Specificity:Human CD208 / DC-LAMP / LAMP3
Application:ICC/IF, IF

ICC/IF: 10-25 μg/mL

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Human LAMP3 Antibody IF Application Image
[Click to enlarge image]
Immunofluorescence staining of Human LAMP3 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human LAMP3 monoclonal antibody (15 µg/ml) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and countstained with DAPI (blue). Positive staining was localized to cytoplasm.
CD208 / DC-LAMP / LAMP3 Antibody, Rabbit MAb, Immunofluorescence
Other LAMP3 Antibody Products
CD208/DC-LAMP Background

Dendritic cell-lysosomal associated membrane protein (DC-LAMP)/CD208, also known as LAMP3, is a member of the lysosomal associated membrane protein (LAMP) family, which is specifically expressed by human dendritic cells (DCs) upon activation and therefore serves as marker of human DC maturation. Confocal and immunoelectron microscopy showed that mouse DC-LAMP protein co-localizes with lbm180, a specific marker for the limiting membrane of lamellar bodies that contain surfactant protein B. The present study demonstrates that DC-LAMP is constitutively expressed by mouse, sheep, and human type II pneumocytes. DC-LAMP is constitutively expressed in normal type II pneumocytes. DC-LAMP is detected first in activated human DC within MHC class II molecules-containing compartments just before the translocation of MHC class II-peptide complexes to the cell surface, suggesting a possible involvement in this process. Furthermore, overexpression of LAMP3 is actively involved in tumor invasion through increased migration into lymph-vascular spaces.

Human CD208/DC-LAMP References
  • Salaun B, et al. (2003) Cloning and characterization of the mouse homologue of the human dendritic cell maturation marker CD208/DC-LAMP. Eur J Immunol. 33(9): 2619-29.
  • Salaun B, et al. (2004) CD208/dendritic cell-lysosomal associated membrane protein is a marker of normal and transformed type II pneumocytes. Am J Pathol. 164(3): 861-71.
  • Ishigami S, et al. (2010) Prognostic value of CD208-positive cell infiltration in gastric cancer. Cancer Immunol Immunother. 59(3): 389-95.
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