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PD1 / PDCD1 / CD279 Antibody, Mouse MAb

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PDCD1Antibody Product Information
Immunogen:Recombinant Human PD1 / PDCD1 / CD279 protein (Catalog#10377-H08H)
Clone ID:F07
Ig Type:Mouse IgG1
Concentration:
Formulation:0.2 μm filtered solution in PBS
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human PD1 / PDCD1 / CD279 (rh PD1 / PDCD1 / CD279; Catalog#10377-H08H; NP_005009.2; Met1-Gln167). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
PDCD1Antibody Usage Guide
Specificity:Human PD1 / PDCD1 / CD279
Application:IHC-P

IHC-P: 10-30 μg/mL

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Images
PD1 / PDCD1 / CD279 Antibody, Mouse MAb, Immunochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of human PD1 in human tonsil with mouse monoclonal antibody (15 µg/mL,formalin-fixed paraffin embedded sections).
PD1 / PDCD1 / CD279 Antibody, Mouse MAb, Immunochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of human PD1 in human spleen with mouse monoclonal antibody (15 µg/mL,formalin-fixed paraffin embedded sections).
PD1 / PDCD1 / CD279 Antibody, Mouse MAb, Immunochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of human PD1 in human lymphnode with mouse monoclonal antibody (15 µg/mL,formalin-fixed paraffin embedded sections).
Background

Programmed cell death 1, also known as PDCD1, is a type I transmembrane glycoprotein, and is an immunoreceptor belonging to the CD28/CTLA-4 family negatively regulates antigen receptor signaling by recruiting protein tyrosine phosphatase, SHP-2 upon interacting with either of two ligands, PD-L1 or PD-L2. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. PD1 has been suggested to be involved in lymphocyte clonal selection and peripheral tolerance, and thus contributes to the prevention of autoimmune diseases. Furthermore, PD1 is shown to be a regulator of virus-specific CD8+ T cell survival in HIV infection. As a cell surface molecule, PDCD1 regulates the adaptive immune response. Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function.

References
  • James ES, et al. (2005) PDCD1: a tissue-specific susceptibility locus for inherited inflammatory disorders. Genes Immun. 6(5): 430-7.
  • Okazaki T, et al. (2007) PD-1 and PD-1 ligands: from discovery to clinical application. Int Immunol. 19(7): 813-24.
  • del Rio ML, et al. (2008) PD-1/PD-L1, PD-1/PD-L2, and other co-inhibitory signaling pathways in transplantation. Transpl Int. 21(11): 1015-28.
  • Riley JL.(2009) PD-1 signaling in primary T cells. Immunol Rev. 229(1): 114-25.
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    Catalog:10377-MF07-100
    List Price: $230.00  (Save $0.00)
    Price:$230.00      [How to order]
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    Images
    • PD1 / PDCD1 / CD279 Antibody, Mouse MAb, Immunochemistry
    • PD1 / PDCD1 / CD279 Antibody, Mouse MAb, Immunochemistry
    • PD1 / PDCD1 / CD279 Antibody, Mouse MAb, Immunochemistry
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