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CD74 Antibody, Mouse MAb

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CD74Antibody Product Information
Immunogen:Recombinant Human CD74 protein (Catalog#11091-H07H)
Clone ID:01
Ig Type:Mouse IgG1
Concentration:
Formulation:0.2 μm filtered solution in PBS
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD74 (rh CD74; Catalog#11091-H07H; NP_005219; Met668-Ala1210). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
CD74Antibody Usage Guide
Specificity:Human CD74
Application:IHC-P

IHC-P: 0.2-2 μg/mL

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Images
CD74 Antibody, Mouse MAb, Immunochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of human CD74 in human tonsil with mouse monoclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).
CD74 Antibody, Mouse MAb, Immunochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of human CD74 in human colon carcinoma with mouse monoclonal antibody (1µg/mL, formalin-fixed paraffin embedded sections).
CD74 Antibody, Mouse MAb, Immunochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of human CD74 in human appendix with mouse monoclonal antibody (1µg/mL, formalin-fixed paraffin embedded sections).
Background

The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD74, also known as HLA class2 histocompatibility antigen gamma chain and HLA-DR antigens-associated invariant chain, is a polypeptide involved in the formation and transport of MHC class2 protein. CD74 is expressed by B cells, macrophages, and Reed-Sternberg cells. When MHC class 2 protein was in the rough ER, its peptide-binding cleft was blocked by CD74 to prevent it from interacting with the endogenous peptides. CD74 also serves to facilitate MHC class2's export from ER.

References
  • Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
  • Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
  • Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.
  • Moynes R, et al. (1997) A marker to distinguish atypical fibroxanthoma from malignant fibrous histiocytoma. Cancer. 79 (11): 2115-24.
  • Gabrielle FA, et al. (2008) Regulation of dendritic cell migration by CD74, the MHC class 2-associated invariant chain. Science. 322 (5908): 1705-10.
  • Leng L, et al. (2003) MIF signal transduction initiated by binding to CD74. JEM.197 (11): 1467-76.
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    Catalog:11091-MM01-100
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    • CD74 Antibody, Mouse MAb, Immunochemistry
    • CD74 Antibody, Mouse MAb, Immunochemistry
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