|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse SUMO1 ORF mammalian expression plasmid, C-GFPSpark tag||MG53059-ACG|
|Mouse SUMO1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG53059-ACR|
|Mouse SUMO1 ORF mammalian expression plasmid, N-GFPSpark tag||MG53059-ANG|
|Mouse SUMO1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG53059-ANR|
|Mouse SUMO1 ORF mammalian expression plasmid, C-Flag tag||MG53059-CF|
|Mouse SUMO1 ORF mammalian expression plasmid, C-His tag||MG53059-CH|
|Mouse SUMO1 ORF mammalian expression plasmid, C-Myc tag||MG53059-CM|
|Mouse SUMO1 ORF mammalian expression plasmid, C-HA tag||MG53059-CY|
|Mouse SUMO1 Gene cDNA clone plasmid||MG53059-G|
|Mouse SUMO1 ORF mammalian expression plasmid, N-Flag tag||MG53059-NF|
|Mouse SUMO1 ORF mammalian expression plasmid, N-His tag||MG53059-NH|
|Mouse SUMO1 ORF mammalian expression plasmid, N-Myc tag||MG53059-NM|
|Mouse SUMO1 ORF mammalian expression plasmid, N-HA tag||MG53059-NY|
|Mouse SUMO1 natural ORF mammalian expression plasmid||MG53059-UT|
|Learn more about expression Vectors|
Small ubiquitin-like modifier protein (SUMO) modification is a highly dynamic process, catalyzed by SUMO-specific activating (E1), conjugating (E2) and ligating (E3) enzymes, and reversed by a family of SUMO-specific proteases (SENPs). Small ubiquitin-like modifier 1 (SUMO1) is a member of the superfamily of ubiquitin-like proteins. Despite its structural similarity with ubiquitin, SUMO1 does not seem to play any role in protein degradation. SUMO1 plays an important role in modulation of NOX activity required for ROS generation. SUMO1 haploinsufficiency results in cleft lip and palate in animal models. SUMO1 gene variation in human non-syndromic cleft lip with or without cleft palate (NSCLP) development. SUMO-1 may be useful as a novel target for therapy in oral squamous cell carcinoma (SCC) as well as a clinical indicator for tumor recurrence together with Mdm2.