|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse RELA ORF mammalian expression plasmid, C-GFPSpark tag||MG53057-ACG|
|Mouse RELA ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG53057-ACR|
|Mouse RELA ORF mammalian expression plasmid, N-GFPSpark tag||MG53057-ANG|
|Mouse RELA ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG53057-ANR|
|Mouse RELA ORF mammalian expression plasmid, C-Flag tag||MG53057-CF|
|Mouse RELA ORF mammalian expression plasmid, C-His tag||MG53057-CH|
|Mouse RELA ORF mammalian expression plasmid, C-Myc tag||MG53057-CM|
|Mouse RELA ORF mammalian expression plasmid, C-HA tag||MG53057-CY|
|Mouse RELA Gene cDNA clone plasmid||MG53057-G|
|Mouse RELA ORF mammalian expression plasmid, N-Flag tag||MG53057-NF|
|Mouse RELA ORF mammalian expression plasmid, N-His tag||MG53057-NH|
|Mouse RELA ORF mammalian expression plasmid, N-Myc tag||MG53057-NM|
|Mouse RELA ORF mammalian expression plasmid, N-HA tag||MG53057-NY|
|Mouse RELA natural ORF mammalian expression plasmid||MG53057-UT|
|Learn more about expression Vectors|
RELA (v-rel reticuloendotheliosis viral oncogene homolog A), also known as Nuclear factor NF-kappa-B p65 subunit, or Transcription factor p65, is a transcription factor expressed in growth plate chondrocytes where it facilitates chondrogenesis. The v-rel avian reticuloendotheliosis viral oncogene homolog A (RELA) gene encodes the major component of the NF-?B complex. NF-kappaB is a generic name for an evolutionarily conserved transcription-factor system that contributes to the mounting of an effective immune response but is also involved in the regulation of cell proliferation, development, and apoptosis. The implication of NF-kappaB in central biological processes and its extraordinary connectivity to other signaling pathways raise a need for highly controlled regulation of NF-kappaB activity at several levels. The mammalian Rel/NF-kappaB family of transcription factors, including RelA, c-Rel, RelB, NF-kappaB1 (p50 and its precursor p105), and NF-kappaB2 (p52 and its precursor p100), plays a central role in the immune system by regulating several processes ranging from the development and survival of lymphocytes and lymphoid organs to the control of immune responses and malignant transformation.