|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse TMED1 ORF mammalian expression plasmid, C-GFPSpark tag||MG52984-ACG|
|Mouse TMED1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG52984-ACR|
|Mouse TMED1 ORF mammalian expression plasmid, C-Flag tag||MG52984-CF|
|Mouse TMED1 ORF mammalian expression plasmid, C-His tag||MG52984-CH|
|Mouse TMED1 ORF mammalian expression plasmid, C-Myc tag||MG52984-CM|
|Mouse TMED1 ORF mammalian expression plasmid, C-HA tag||MG52984-CY|
|Mouse TMED1 Gene cDNA clone plasmid||MG52984-G|
|Mouse TMED1 ORF mammalian expression plasmid, N-Flag tag||MG52984-NF|
|Mouse TMED1 ORF mammalian expression plasmid, N-His tag||MG52984-NH|
|Mouse TMED1 ORF mammalian expression plasmid, N-Myc tag||MG52984-NM|
|Mouse TMED1 ORF mammalian expression plasmid, N-HA tag||MG52984-NY|
|Mouse TMED1 natural ORF mammalian expression plasmid||MG52984-UT|
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TMED1 belongs to the EMP24/GP25L family. It contains 1 GOLD domain and is widely expressed. TMED1 binds to its receptor IL1RL1 and results in the activation of DNA binding by nuclear factor NF-kappa-B or transcription from the IL8 promoter and most likely requires other proteins to elicit these activities. Dendritic cells from Peyer's patches (but not from spleen) express TMED1 in response to treatment with LPS. TMED1 may play a role in vesicular protein trafficking, mainly in the early secretory pathway. It may act as a cargo receptor at the lumenal side for incorporation of secretory cargo molecules into transport vesicles and may be involved in vesicle coat formation at the cytoplasmic side.