|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse C1D ORF mammalian expression plasmid, C-GFPSpark tag||MG52866-ACG|
|Mouse C1D ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG52866-ACR|
|Mouse C1D ORF mammalian expression plasmid, N-GFPSpark tag||MG52866-ANG|
|Mouse C1D ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG52866-ANR|
|Mouse C1D ORF mammalian expression plasmid, C-Flag tag||MG52866-CF|
|Mouse C1D ORF mammalian expression plasmid, C-His tag||MG52866-CH|
|Mouse C1D ORF mammalian expression plasmid, C-Myc tag||MG52866-CM|
|Mouse C1D ORF mammalian expression plasmid, C-HA tag||MG52866-CY|
|Mouse C1D Gene cDNA clone plasmid||MG52866-G|
|Mouse C1D ORF mammalian expression plasmid, N-Flag tag||MG52866-NF|
|Mouse C1D ORF mammalian expression plasmid, N-His tag||MG52866-NH|
|Mouse C1D ORF mammalian expression plasmid, N-Myc tag||MG52866-NM|
|Mouse C1D ORF mammalian expression plasmid, N-HA tag||MG52866-NY|
|Mouse C1D natural ORF mammalian expression plasmid||MG52866-UT|
|Learn more about expression Vectors|
C1D nuclear receptor corepressor belongs to the C1D family. It is a DNA binding and apoptosis-inducing protein.C1D nuclear receptor corepressorinteracts with TSNAX and DNA-PKcs. It acts as a corepressor for the thyroid hormone receptor. It is thought that C1D nuclear receptor corepressor regulates TRAX/Translin complex formation. It is expressed in kidney, heart, brain, spleen, lung, testis, liver and small intestine. It plays a role in the recruitment of the RNA exosome complex to pre-rRNA to mediate the 3'-5' end processing of the 5.8S rRNA; this function may include MPHOSPH6. It potentiates transcriptional repression by NR1D1 and THRB. C1D nuclear receptor corepressorcan activate PRKDC not only in the presence of linear DNA but also in the presence of supercoiled DNA. It also can induce apoptosis in a p53/TP53 dependent manner.