|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse GSTM2 ORF mammalian expression plasmid, C-GFPSpark tag||MG52883-ACG|
|Mouse GSTM2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG52883-ACR|
|Mouse GSTM2 ORF mammalian expression plasmid, N-GFPSpark tag||MG52883-ANG|
|Mouse GSTM2 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG52883-ANR|
|Mouse GSTM2 ORF mammalian expression plasmid, C-Flag tag||MG52883-CF|
|Mouse GSTM2 ORF mammalian expression plasmid, C-His tag||MG52883-CH|
|Mouse GSTM2 ORF mammalian expression plasmid, C-Myc tag||MG52883-CM|
|Mouse GSTM2 ORF mammalian expression plasmid, C-HA tag||MG52883-CY|
|Mouse GSTM2 Gene cDNA clone plasmid||MG52883-G|
|Mouse GSTM2 ORF mammalian expression plasmid, N-Flag tag||MG52883-NF|
|Mouse GSTM2 ORF mammalian expression plasmid, N-His tag||MG52883-NH|
|Mouse GSTM2 ORF mammalian expression plasmid, N-Myc tag||MG52883-NM|
|Mouse GSTM2 ORF mammalian expression plasmid, N-HA tag||MG52883-NY|
|Mouse GSTM2 natural ORF mammalian expression plasmid||MG52883-UT|
|Learn more about expression Vectors|
Glutathione S-transferase Mu 2, also known as GST class-mu 2, GSTM2-2 and GSTM2, is a cytoplasm protein which belongs to the GST superfamily and Mu family. GSTM2 / GST4 contains one GST C-terminal domain and one GST N-terminal domain. The glutathione S-transferases (GSTs) are a multigene family of enzymes largely involved in the detoxification of chemicals. Eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. Butyrate, an important luminal component produced from fermentation of dietary fibers, is an efficient inducer of GSTs and especially of GSTM2. Butyrate may act chemoprotectively by increasing detoxification capabilities in the colon mucosa.