|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse ENO3 ORF mammalian expression plasmid, C-GFPSpark tag||MG52862-ACG|
|Mouse ENO3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG52862-ACR|
|Mouse ENO3 ORF mammalian expression plasmid, C-Flag tag||MG52862-CF|
|Mouse ENO3 ORF mammalian expression plasmid, C-His tag||MG52862-CH|
|Mouse ENO3 ORF mammalian expression plasmid, C-Myc tag||MG52862-CM|
|Mouse ENO3 ORF mammalian expression plasmid, C-HA tag||MG52862-CY|
|Mouse ENO3 Gene cDNA clone plasmid||MG52862-G|
|Mouse ENO3 ORF mammalian expression plasmid, N-Flag tag||MG52862-NF|
|Mouse ENO3 ORF mammalian expression plasmid, N-His tag||MG52862-NH|
|Mouse ENO3 ORF mammalian expression plasmid, N-Myc tag||MG52862-NM|
|Mouse ENO3 ORF mammalian expression plasmid, N-HA tag||MG52862-NY|
|Mouse ENO3 natural ORF mammalian expression plasmid||MG52862-UT|
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ENO3 is one of the three enolase isoenzymes found in mammals. As a homodimer, ENO3 is found in skeletal muscle cells in the adult. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in ENO3 gene can be associated with metabolic myopathies that may result from decreased stability of the enzyme. Two transcripts have been identified for ENO3 gene that differ only in their 5' UTR. ENO3 may play a role in muscle development and regeneration. It appears to have a function in striated muscle development and regeneration.