|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse LYPLA2 ORF mammalian expression plasmid, C-GFPSpark tag||MG52835-ACG|
|Mouse LYPLA2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG52835-ACR|
|Mouse LYPLA2 ORF mammalian expression plasmid, C-Flag tag||MG52835-CF|
|Mouse LYPLA2 ORF mammalian expression plasmid, C-His tag||MG52835-CH|
|Mouse LYPLA2 ORF mammalian expression plasmid, C-Myc tag||MG52835-CM|
|Mouse LYPLA2 ORF mammalian expression plasmid, C-HA tag||MG52835-CY|
|Mouse LYPLA2 Gene cDNA clone plasmid||MG52835-G|
|Mouse LYPLA2 ORF mammalian expression plasmid, N-Flag tag||MG52835-NF|
|Mouse LYPLA2 ORF mammalian expression plasmid, N-His tag||MG52835-NH|
|Mouse LYPLA2 ORF mammalian expression plasmid, N-Myc tag||MG52835-NM|
|Mouse LYPLA2 ORF mammalian expression plasmid, N-HA tag||MG52835-NY|
|Mouse LYPLA2 natural ORF mammalian expression plasmid||MG52835-UT|
|Learn more about expression Vectors|
Lysophospholipase II (LYPLA2, LPL-II, or LysoPLA II), also known as Acyl-protein thioesterase 2 (APT-2), belongs to the AB hydrolase 2 family. This enzyme has lysophospholipase activity, and may hydrolyze fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS. Acyl-protein thioesterase 1 (APT-1) and Acyl-protein thioesterase 2 (APT-2) are cytosolic lysophospholipid hydrolyzing enzymes. The serum activity of APT-1 may play an important role in determination of the concentration of des-acyl ghrelin in circulation, especially under septic inflammation. APT-2/LYPLA2 is expressed both in CHO-K1 and HeLa cells and its overexpression increased the deacylation rate of single acylated GAP-43 and affected the steady-state localization of diacylated GAP-43 and H-Ras. Thus, the results demonstrate that APT-2/LYPLA2 is the protein thioesterase involved in the acylation/deacylation cycle operating in GAP-43 subcellular distribution.