|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse LYPLA2 ORF mammalian expression plasmid, C-GFPSpark tag||MG52835-ACG|
|Mouse LYPLA2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG52835-ACR|
|Mouse LYPLA2 ORF mammalian expression plasmid, C-Flag tag||MG52835-CF|
|Mouse LYPLA2 ORF mammalian expression plasmid, C-His tag||MG52835-CH|
|Mouse LYPLA2 ORF mammalian expression plasmid, C-Myc tag||MG52835-CM|
|Mouse LYPLA2 ORF mammalian expression plasmid, C-HA tag||MG52835-CY|
|Mouse LYPLA2 Gene cDNA clone plasmid||MG52835-G|
|Mouse LYPLA2 ORF mammalian expression plasmid, N-Flag tag||MG52835-NF|
|Mouse LYPLA2 ORF mammalian expression plasmid, N-His tag||MG52835-NH|
|Mouse LYPLA2 ORF mammalian expression plasmid, N-Myc tag||MG52835-NM|
|Mouse LYPLA2 ORF mammalian expression plasmid, N-HA tag||MG52835-NY|
|Mouse LYPLA2 natural ORF mammalian expression plasmid||MG52835-UT|
|Learn more about expression Vectors|
Lysophospholipase II (LYPLA2, LPL-II, or LysoPLA II), also known as Acyl-protein thioesterase 2 (APT-2), belongs to the AB hydrolase 2 family. This enzyme has lysophospholipase activity, and may hydrolyze fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS. Acyl-protein thioesterase 1 (APT-1) and Acyl-protein thioesterase 2 (APT-2) are cytosolic lysophospholipid hydrolyzing enzymes. The serum activity of APT-1 may play an important role in determination of the concentration of des-acyl ghrelin in circulation, especially under septic inflammation. APT-2/LYPLA2 is expressed both in CHO-K1 and HeLa cells and its overexpression increased the deacylation rate of single acylated GAP-43 and affected the steady-state localization of diacylated GAP-43 and H-Ras. Thus, the results demonstrate that APT-2/LYPLA2 is the protein thioesterase involved in the acylation/deacylation cycle operating in GAP-43 subcellular distribution.