|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat HRG ORF mammalian expression plasmid, C-GFPSpark tag||RG81643-ACG|
|Rat HRG ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG81643-ACR|
|Rat HRG ORF mammalian expression plasmid, N-GFPSpark tag||RG81643-ANG|
|Rat HRG ORF mammalian expression plasmid, N-OFPSpark / RFP tag||RG81643-ANR|
|Rat HRG ORF mammalian expression plasmid, C-Flag tag||RG81643-CF|
|Rat HRG ORF mammalian expression plasmid, C-His tag||RG81643-CH|
|Rat HRG ORF mammalian expression plasmid, C-Myc tag||RG81643-CM|
|Rat HRG ORF mammalian expression plasmid, C-HA tag||RG81643-CY|
|Rat HRG Gene cDNA clone plasmid||RG81643-G|
|Rat HRG ORF mammalian expression plasmid, N-Flag tag||RG81643-NF|
|Rat HRG ORF mammalian expression plasmid, N-His tag||RG81643-NH|
|Rat HRG ORF mammalian expression plasmid, N-Myc tag||RG81643-NM|
|Rat HRG ORF mammalian expression plasmid, N-HA tag||RG81643-NY|
|Rat HRG natural ORF mammalian expression plasmid||RG81643-UT|
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Histidine-rich glycoprotein, also known as HRG and HPRG, is a glycoprotein located in plasma and platelets, and contains an unusually large amount of histidine and proline. In human, five distinct domains are recognized in the mature HPRG molecule. There are two N-terminal cystatin-like modules (aa 19 - 254) and one His-Pro-rich region (aa 350 - 497) that is flanked by two Pro-rich segments (aa 276 - 321 and 498 - 525). The His-Pro-rich region contains 10 tandem repeats with an HHPHG motif, and the N- and C-termini are linked by a disulfide bond. The specific functions of HRG remain unclear, but it is known that the protein binds heme, dyes and divalent metal ions. It inhibits rosette formation and interacts with heparin, thrombospondin and plasminogen. Two of the protein's effects, the inhibition of fibrinolysis and the reduction of inhibition of coagulation, indicate a potential prothrombotic effect. HPRG is evolutionarily, functionally and structurally related to cleaved high molecular weight kininogen (HKa), an anti-angiogenic polypeptide that stimulates apoptosis of proliferating endothelial cells through binding to cell-surface tropomyosin. The antiangiogenic activity of the multidomain plasma protein HPRG is localized to its histidine-proline-rich (H/P) domain and has recently been shown to be mediated, at least partially, through binding to cell-surface tropomyosin in fibroblast growth factor-2-activated endothelial cells.