|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat FETUB ORF mammalian expression plasmid, C-GFPSpark tag||RG81641-ACG|
|Rat FETUB ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG81641-ACR|
|Rat FETUB ORF mammalian expression plasmid, N-GFPSpark tag||RG81641-ANG|
|Rat FETUB ORF mammalian expression plasmid, N-OFPSpark / RFP tag||RG81641-ANR|
|Rat FETUB ORF mammalian expression plasmid, C-Flag tag||RG81641-CF|
|Rat FETUB ORF mammalian expression plasmid, C-His tag||RG81641-CH|
|Rat FETUB ORF mammalian expression plasmid, C-Myc tag||RG81641-CM|
|Rat FETUB ORF mammalian expression plasmid, C-HA tag||RG81641-CY|
|Rat FETUB Gene cDNA clone plasmid||RG81641-G|
|Rat FETUB ORF mammalian expression plasmid, N-Flag tag||RG81641-NF|
|Rat FETUB ORF mammalian expression plasmid, N-His tag||RG81641-NH|
|Rat FETUB ORF mammalian expression plasmid, N-Myc tag||RG81641-NM|
|Rat FETUB ORF mammalian expression plasmid, N-HA tag||RG81641-NY|
|Rat FETUB natural ORF mammalian expression plasmid||RG81641-UT|
|Learn more about expression Vectors|
Fetuin-B, also known as Fetuin-like protein IRL685 and FETUB, is a secreted protein which belongs to the fetuin family. Fetuin-B / FETUB contains two cystatin domains. Fetuin-B is a member of the fetuin family, part of the cystatin superfamily of cysteine protease inhibitors. Fetuins have been implicated in several diverse functions, including osteogenesis and bone resorption. Fetuin-A has been identified as a major protein during fetal life and is also involved in important functions such as protease inhibitory activities and development-associated regulation of calcium metabolism and osteogenesis. Fetuin-A is a key partner in the recovery phase of an acute inflammatory response. Fetuin-B / FETUB is found at least in human and rodents. It is unambiguously a paralogue of Fetuin-A. Fetuin-A and Fetuin-B exhibit significant differences at the amino acid sequence level, notably including variations with respect to the archetypal fetuin-specific signature.