|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse AFP ORF mammalian expression plasmid, C-GFPSpark tag||MG52903-ACG|
|Mouse AFP ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG52903-ACR|
|Mouse AFP ORF mammalian expression plasmid, C-Flag tag||MG52903-CF|
|Mouse AFP ORF mammalian expression plasmid, C-His tag||MG52903-CH|
|Mouse AFP ORF mammalian expression plasmid, C-Myc tag||MG52903-CM|
|Mouse AFP ORF mammalian expression plasmid, C-HA tag||MG52903-CY|
|Mouse AFP Gene cDNA clone plasmid||MG52903-G|
|Mouse AFP ORF mammalian expression plasmid, N-Flag tag||MG52903-NF|
|Mouse AFP ORF mammalian expression plasmid, N-His tag||MG52903-NH|
|Mouse AFP ORF mammalian expression plasmid, N-Myc tag||MG52903-NM|
|Mouse AFP ORF mammalian expression plasmid, N-HA tag||MG52903-NY|
|Mouse AFP natural ORF mammalian expression plasmid||MG52903-UT|
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Alpha-fetoprotein (AFP) is classified as a member of the albuminoid gene superfamily consisting of albumin, AFP, vitamin D (Gc) protein, and alpha-albumin. AFP is a glycoprotein of 591 amino acids and a carbohydrate moiety. AFP is one of the several embryo-specific proteins and is a dominant serum protein as early in human embryonic life as one month, when albumin and transferrin are present in relatively small amounts. It is first synthesized in the human by the yolk sac and liver(1-2 months) and subsequently predominantly in the liver. A small amount of AFP is produced by the GI tract of the human conceptus. It has been proved that AFP may reappear in the serum in elevated amounts in adult life in association with normal restorative processes and with malignant growth. Alpha-fetoprotein (AFP) is a specific marker for hepatocellular carcinoma (HCC), teratoblastomas, and neural tube defect (NTD).