|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Rat HRG ORF mammalian expression plasmid, C-GFPSpark tag||RG81643-ACG|
|Rat HRG ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG81643-ACR|
|Rat HRG ORF mammalian expression plasmid, N-GFPSpark tag||RG81643-ANG|
|Rat HRG ORF mammalian expression plasmid, N-OFPSpark / RFP tag||RG81643-ANR|
|Rat HRG ORF mammalian expression plasmid, C-Flag tag||RG81643-CF|
|Rat HRG ORF mammalian expression plasmid, C-His tag||RG81643-CH|
|Rat HRG ORF mammalian expression plasmid, C-Myc tag||RG81643-CM|
|Rat HRG ORF mammalian expression plasmid, C-HA tag||RG81643-CY|
|Rat HRG Gene cDNA clone plasmid||RG81643-G|
|Rat HRG ORF mammalian expression plasmid, N-Flag tag||RG81643-NF|
|Rat HRG ORF mammalian expression plasmid, N-His tag||RG81643-NH|
|Rat HRG ORF mammalian expression plasmid, N-Myc tag||RG81643-NM|
|Rat HRG ORF mammalian expression plasmid, N-HA tag||RG81643-NY|
|Rat HRG natural ORF mammalian expression plasmid||RG81643-UT|
|Learn more about expression Vectors|
Histidine-rich glycoprotein, also known as HRG and HPRG, is a glycoprotein located in plasma and platelets, and contains an unusually large amount of histidine and proline. In human, five distinct domains are recognized in the mature HPRG molecule. There are two N-terminal cystatin-like modules (aa 19 - 254) and one His-Pro-rich region (aa 350 - 497) that is flanked by two Pro-rich segments (aa 276 - 321 and 498 - 525). The His-Pro-rich region contains 10 tandem repeats with an HHPHG motif, and the N- and C-termini are linked by a disulfide bond. The specific functions of HRG remain unclear, but it is known that the protein binds heme, dyes and divalent metal ions. It inhibits rosette formation and interacts with heparin, thrombospondin and plasminogen. Two of the protein's effects, the inhibition of fibrinolysis and the reduction of inhibition of coagulation, indicate a potential prothrombotic effect. HPRG is evolutionarily, functionally and structurally related to cleaved high molecular weight kininogen (HKa), an anti-angiogenic polypeptide that stimulates apoptosis of proliferating endothelial cells through binding to cell-surface tropomyosin. The antiangiogenic activity of the multidomain plasma protein HPRG is localized to its histidine-proline-rich (H/P) domain and has recently been shown to be mediated, at least partially, through binding to cell-surface tropomyosin in fibroblast growth factor-2-activated endothelial cells.